低温電子顕微鏡法
出典: フリー百科事典『ウィキペディア（Wikipedia）』 (2023/07/15 23:44 UTC 版)
低温電子顕微鏡法（ていおんでんしけんびきょうほう、Cryo-electron microscopy、cryo-EM、クライオ電子顕微鏡法）は透過型電子顕微鏡法の一種で、試料を低温（多くの場合液体窒素の温度）において解析する手法である[1]。構造生物学や細胞生物学の分野において用いられる[2]。
- ^ a b c Kuehlbrandt, Werner (2014). “Cryo-EM enters a new era”. eLife 3: e03678. doi:10.7554/elife.03678. PMC 4131193. PMID 25122623.
- ^ a b Callaway, Ewen (2015). “The revolution will not be crystallized: A new method sweeps through structural biology”. Nature 525 (7568): 172–4. Bibcode: 2015Natur.525..172C. doi:10.1038/525172a. PMID 26354465.
- ^ Zivanov, Jasenko; Nakane, Takanori; Forsberg, Björn O.; Kimanius, Dari; Hagen, Wim Jh; Lindahl, Erik; Scheres, Sjors Hw (11 09, 2018). “New tools for automated high-resolution cryo-EM structure determination in RELION-3”. eLife 7. doi:10.7554/eLife.42166. ISSN 2050-084X. PMC 6250425. PMID 30412051.
- ^ Adrian, Marc; Dubochet, Jacques; Lepault, Jean; McDowall, Alasdair W. (1984). “Cryo-electron microscopy of viruses”. Nature 308 (5954): 32–6. Bibcode: 1984Natur.308...32A. doi:10.1038/308032a0. PMID 6322001.
- ^ Grant J. Jensen: Cryo-EM Part B: 3-D Reconstruction. In: Methods in Enzymology. Band 482, Academic Press, 2010, ISBN 978-0-123-84992-2. S. 211.
- ^ Joachim Frank: Electron Tomography. Springer Science & Business Media, 2008, ISBN 978-0-387-69008-7, S. 50
- ^ R. A. Crowther: The Resolution Revolution: Recent Advances In cryoEM. In: Methods in Enzymology, Band 579, Academic Press, 2016, ISBN 978-0-128-05435-2, S. 369.
- ^ Fu, Ziao; Kaledhonkar, Sandip; Borg, Anneli; Sun, Ming; Chen, Bo; Grassucci, Robert A.; Ehrenberg, Måns; Frank, Joachim (2016). “Key Intermediates in Ribosome Recycling Visualized by Time-Resolved Cryoelectron Microscopy”. Structure 24 (12): 2092–2101. doi:10.1016/j.str.2016.09.014. PMC 5143168. PMID 27818103.
- ^ Feng, Xiangsong; Fu, Ziao; Kaledhonkar, Sandip; Jia, Yuan; Shah, Binita; Jin, Amy; Liu, Zheng; Sun, Ming et al. (2017). “A Fast and Effective Microfluidic Spraying-Plunging Method for High-Resolution Single-Particle Cryo-EM”. Structure 25 (4): 663–670.e3. doi:10.1016/j.str.2017.02.005. PMC 5382802. PMID 28286002.
- ^ Chen, Bo; Kaledhonkar, Sandip; Sun, Ming; Shen, Bingxin; Lu, Zonghuan; Barnard, David; Lu, Toh-Ming; Gonzalez, Ruben L. et al. (2015). “Structural Dynamics of Ribosome Subunit Association Studied by Mixing-Spraying Time-Resolved Cryogenic Electron Microscopy”. Structure 23 (6): 1097–105. doi:10.1016/j.str.2015.04.007. PMC 4456197. PMID 26004440.
- ^ Levin, Barnaby D.A.; Zachman, Michael J.; Werner, Jörg G.; Sahore, Ritu; Nguyen, Kayla X.; Han, Yimo; Xie, Baoquan; Ma, Lin et al. (2017). “Characterization of Sulfur and Nanostructured Sulfur Battery Cathodes in Electron Microscopy Without Sublimation Artifacts”. Microscopy and Microanalysis 23 (1): 155–162. Bibcode: 2017MiMic..23..155L. doi:10.1017/S1431927617000058. PMID 28228169.
- ^ “The 2017 Nobel Prize in Chemistry - Press Release”. www.nobelprize.org (2017年10月4日). 2017年10月4日閲覧。
- ^ “Nobel Prize in Chemistry Awarded for Cryo-Electron Microscopy”. The New York Times. (2017年10月4日) 2017年10月4日閲覧。
[続きの解説]
「低温電子顕微鏡法」の続きの解説一覧
- 1 低温電子顕微鏡法とは
- 2 低温電子顕微鏡法の概要
- 3 受賞
- 低温電子顕微鏡法のページへのリンク
