ID_REF | Feature number |
PositionX | Found X coordinate of feature centroid in pixels |
PositionY | Found Y coordinate of feature centroid in pixels |
VALUE | log(REDsignal/GREENsignal) per feature (processed signals used) |
LogRatioError | error of the log ratio calculated according to the error model chosen |
PValueLogRatio | Significance level of the Log Ratio computed for a feature |
gSurrogateUsed | The green surrogate value used |
rSurrogateUsed | The red surrogate value used |
gIsFound | A boolean used to flag found (strong) features.The flag is applied independently in each channel. A feature is considered found if the found spot centroid is within the bounds of the spot deviation limit with respect to corresponding nominal centroid. |
rIsFound | A boolean used to flag found (strong) features.The flag is applied independently in each channel. A feature is considered found if the found spot centroid is within the bounds of the spot deviation limit with respect to corresponding nominal centroid. |
gProcessedSignal | Dye-normalized signal after surrogate algorithm, green channel, used for computation of log ratio |
rProcessedSignal | Dye-normalized signal after surrogate algorithm, red channel, used for computation of log ratio |
gProcessedSigError | Standard error of propagated feature signal, green channel |
rProcessedSigError | Standard error of propagated feature signal, red channel |
gNumPixOLHi | Number of outlier pixels per feature with intensity > upper threshold set via the pixel outlier rejection method. The number is computed independently in each channel. These pixels are omitted from all subsequent calculations. |
rNumPixOLHi | Number of outlier pixels per feature with intensity > upper threshold set via the pixel outlier rejection method. The number is computed independently in each channel. These pixels are omitted from all subsequent calculations. |
gNumPixOLLo | Number of outlier pixels per feature with intensity < lower threshold set via the pixel outlier rejection method. The number is computed independently in each channel. NOTE: The pixel outlier method is the ONLY step that removes data in Feature Extraction. |
rNumPixOLLo | Number of outlier pixels per feature with intensity < lower threshold set via the pixel outlier rejection method. The number is computed independently in each channel. NOTE: The pixel outlier method is the ONLY step that removes data in Feature Extraction. |
gNumPix | Total number of pixels used to compute feature statistics; ie.total number of inlier pixels/per spot; same in both channels |
rNumPix | Total number of pixels used to compute feature statistics; ie.total number of inlier pixels/per spot; same in both channels |
gMeanSignal | Raw mean signal of feature in green channel (inlier pixels) |
rMeanSignal | Raw mean signal of feature in red channel (inlier pixels) |
gMedianSignal | Raw median signal of feature in green channel (inlier pixels) |
rMedianSignal | Raw median signal of feature in red channel (inlier pixels) |
gPixSDev | Standard deviation of all inlier pixels per feature; this is computed independently in each channel |
rPixSDev | Standard deviation of all inlier pixels per feature; this is computed independently in each channel |
gBGNumPix | Total Number of pixels used to compute Local BG statistics per spot; ie.total number of BG inlier pixels; same in both channels |
rBGNumPix | Total Number of pixels used to compute Local BG statistics per spot; ie.total number of BG inlier pixels; same in both channels |
gBGMeanSignal | Mean local background signal (local to corresponding feature) computed per channel (inlier pixels) |
rBGMeanSignal | Mean local background signal (local to corresponding feature) computed per channel (inlier pixels) |
gBGMedianSignal | Median local background signal (local to corresponding feature) computed per channel (inlier pixels) |
rBGMedianSignal | Median local background signal (local to corresponding feature) computed per channel (inlier pixels) |
gBGPixSDev | Standard deviation of all inlier pixels per Local BG of each feature, computed independently in each channel |
rBGPixSDev | Standard deviation of all inlier pixels per Local BG of each feature, computed independently in each channel |
gNumSatPix | Total number of saturated pixels per feature, computed per channel |
rNumSatPix | Total number of saturated pixels per feature, computed per channel |
gIsSaturated | Boolean flag indicating if a feature is saturated or not |
rIsSaturated | Boolean flag indicating if a feature is saturated or not |
PixCorrelation | Ratio of estimated feature covariance in RedGreen space to product of feature Standard Deviation in Red Green space. |
BGPixCorrelation | Ratio of estimated background covariance in RedGreen space to product of background Standard Deviation in Red Green space. |
gIsFeatNonUnifOL | Boolean flag indicating if a feature is NonUniformity Outlier or not Green Channel |
rIsFeatNonUnifOL | Boolean flag indicating if a feature is NonUniformity Outlier or not Red Channel |
gIsBGNonUnifOL | Boolean flag indicating if background is NonUniformity Outlier or not Green Channel |
rIsBGNonUnifOL | Boolean flag indicating if background is NonUniformity Outlier or not Red Channel |
gIsFeatPopnOL | Boolean flag indicating if a feature is a Population Outlier or not for Green Channel. Probes with replicate features on a microarray are examined using population statistics |
rIsFeatPopnOL | Boolean flag indicating if a feature is a Population Outlier or not for Red Channe. Probes with replicate features on a microarray are examined using population statistics |
gIsBGPopnOL | Boolean flag indicating if background is a Population Outlier or not for Green Channel. |
rIsBGPopnOL | Boolean flag indicating if background is a Population Outlier or not for Red Channel. |
IsManualFlag | Manual Flag |
gBGSubSignal | The net g signal following the subtraction of the background from the raw mean g signal |
rBGSubSignal | The net r signal following the subtraction of the background from the raw mean r signal |
gBGSubSigError | Propagated standard error as computed on net g background subtracted signal |
rBGSubSigError | Propagated standard error as computed on net r background subtracted signal |
BGSubSigCorrelation | Ratio of estimated background subtracted feature signal covariance in RG space to product of background subtracted feature Standard Deviation in RG space |
gIsPosAndSignif | Boolean flag indicating if the mean signal of a feature is greater than the corresponding background (selected by user) and if this difference is significant |
rIsPosAndSignif | Boolean flag indicating if the mean signal of a feature is greater than the corresponding background (selected by user) and if this difference is significant |
gPValFeatEqBG | P-value from t-test of significance between g Mean signal and g background (selected by user) |
rPValFeatEqBG | P-value from t-test of significance between r Mean signal and r background (selected by user) |
gNumBGUsed | Number of local background regions or features used to calculate the background subtraction on this feature g channel. |
rNumBGUsed | Number of local background regions or features used to calculate the background subtraction on this feature r channel. |
gIsWellAboveBG | Boolean flag indicating if a feature is WellAbove Background or not |
rIsWellAboveBG | Boolean flag indicating if a feature is WellAbove Background or not |
IsUsedBGAdjust | A boolean used to flag features used for computation of global BG offset |
gBGUsed | Background value (after global background adjust if turned ON) subtracted from the raw mean signal to generate the BG subtracted signal; this value is computed per channel. If global BG subtraction is used the column is identical for every feature in a given channel |
rBGUsed | Background value (after global background adjust if turned ON) subtracted from the raw mean signal to generate the BG subtracted signal; this value is computed per channel. If global BG subtraction is used the column is identical for every feature in a given channel |
gBGSDUsed | Standard deviation of background used in g channel |
rBGSDUsed | Standard deviation of background used in r channel |
IsNormalization | A boolean flag which indicates if a feature is used to measure dye bias |
gDyeNormSignal | The dye-normalized signal in the indicated channel |
rDyeNormSignal | The dye-normalized signal in the indicated channel |
gDyeNormError | The standard error associated with the dye normalized signal |
rDyeNormError | The standard error associated with the dye normalized signal |
DyeNormCorrelation | Dye-normalized red and green pixel correlation |
ErrorModel | Indicates the error model that you chose for Feature Extraction or that the software uses if you have chosen the “Most Conservative” option |