銀染色
(Silver staining から転送)
出典: フリー百科事典『ウィキペディア（Wikipedia）』 (2023/03/27 18:26 UTC 版)
銀染色（ぎんせんしょく、英: silver staining）とは、組織切片の顕微鏡解析やポリアクリルアミドゲル電気泳動、DGGEにおいて、研究対象の外観を選択的に変化させて可視化するために銀を用いる手法のことである。
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- ^ “Über Probleme der quantitiven Auswertung der mit physikalischer Entwicklung versilberten Agarelektrophoretogramme”. Clin. Chim. Acta 47 (3): 425–436. (1973). doi:10.1016/0009-8981(73)90276-3. PMID 4744834.
- ^ Switzer RC 3rd, Merril CR, Shifrin S (Sep 1979). “A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels.”. Anal. Biochem. 98 (1): 231–237. doi:10.1016/0003-2697(79)90732-2. PMID 94518.
- ^ “Free SH-groups are important for the polychromatic staining of proteins with silver nitrate”. Neuhof V (ed)Electrophoresis '84, Verlag Chemie Weinheim 1984: 328–330. (1984).
- ^ “A modification of the staining technique of reticular fibres for image analysis of the cardiac collagen network”. Cardiovasc. Pathol. 13 (4): 213–20. (2004). doi:10.1016/S1054-8807(03)00153-4. PMID 15210137.
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- ^ L. Kerényi, F. Gallyas: [Errors in quantitative estimations on agar electrophoresis using silver stain]. In: Clinica Chimica Acta. Band 47, Nummer 3, September 1973, S. 425–436, PMID 4744834.
- ^ C. R. Merril, R. C. Switzer, M. L. Van Keuren: Trace polypeptides in cellular extracts and human body fluids detected by two-dimensional electrophoresis and a highly sensitive silver stain. In: Proc Natl Acad Sci U S A. 76(9), 1979, S. 4335–4339. PMID 92027.
- ^ R. C. Switzer, C. R. Merril, S. Shifrin (September 1979), “A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels” (German), Anal Biochem 98 (1): 231–237, doi:10.1016/0003-2697(79)90732-2, PMID 94518
- ^ T. Rabilloud et. al.: Improvement and simplification of low-background silver staining of proteins by using sodium dithionite. In: Electrophoresis. 9(6), 1998, S. 288–291. PMID 2466660.
- ^ T. Rabilloud: A comparison between low background silver diammine and silver nitrate protein stains. In: Electrophoresis. 13, 1992, S. 429–439. PMID 1425556.
- ^ C. Lelong, M. Chevallet, S. Luche, T. Rabilloud: Silver staining of proteins in 2DE gels. In: Methods Mol Biol. 519, 2009, S. 339–350. PMID 19381593.
- ^ H. Blum, H. Beier, H. J. Gross: Improved silver staining of plant protein, RNA & DNA in PAA gels. In: Electrophoresis. 8, 1987, S. 93–99.
- ^ G. Dubray, G. Bezard: A highly sensitive periodic acid-silver stain for 1,2-diol groups of glycoproteins and polysaccharides in polyacrylamide gels. In: Anal. Biochem.. 119(2), 1982, S. 325–329. PMID 6176144.
- ^ E. Hempelmann, M. Schulze, O. Götze: Free SH-groups are important for the polychromatic staining of proteins with silver nitrate. In: V. Neuhof (Editor): Electrophoresis. Verlag Chemie, Weinheim, 1984, pp. 328–330.
- ^ D. Ochs: Protein contaminants of sodium dodecyl sulfate polyacrylamide gels. In: Analytical biochemistry. Vol. 135, Number 2, December 1983, pp. 470-474, PMID 6197906.
- ^ “Characterization of membrane proteins by polychromatic silver staining”. Hoppe-Seyler's Z Physiol Chem 365: 241–242. (1984).
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