Nucleotide sequence of human monocyte interleukin 1 precursor cDNA.
Abstract
Interleukin 1 (IL-1) is a protein with several biological activities regulating host defense and immune responses. We report here the isolation of human IL-1 cDNA. It encodes a precursor polypeptide of 269 amino acids (30,747 Mr). mRNA isolated by hybridization to this cDNA was translated in a reticulocyte cell-free system, yielding immunoprecipitable IL-1. Furthermore, this hybrid-selected mRNA was injected into Xenopus laevis oocytes, which subsequently secreted biologically active IL-1. The cDNA nucleotide sequence suggests that IL-1 is initially translated as a precursor molecule that is subsequently processed into the 15,000-20,000 Mr protein usually associated with IL-1 activity.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- December 1984
- DOI:
- 10.1073/pnas.81.24.7907
- Bibcode:
- 1984PNAS...81.7907A