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Flavobacterium ajazii sp. nov., Isolated from Seaweed of Gouqi Island, China

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Abstract

A novel bacterium designated SSM4.2T was isolated from seaweed of Gouqi Island, which is the center of the Zhoushan fishing ground in the East China Sea. Strain SSM4.2T was Gram-stain-negative, bright yellow-pigmented, short rod-shaped, non-flagellated, non-spore forming, aerobic and motile by gliding. Growth was observed at 4–37 °C (optimum 25–30 °C), pH 6.0–8.0 (optimum pH 7.0) and 0–2.0% (w/v) NaCl (optimum 0%) concentration. The strain was catalase- and oxidase-positive. Menaquinone-6 (MK-6) was found as the sole respiratory quinone and zeaxanthin as the main carotenoid pigment. The predominant fatty acids (≥ 10%) were iso-C15:0, iso-C15:1 G, iso-C17:0 3-OH and summed feature 3 (C16:1 ω7c /C16:1 ω6c). The major polar lipid was phosphatidylethanolamine (PE). The genome size was 5.7 Mbp. The DNA G + C content was 34.1 mol%. 16S rRNA gene sequence revealed that strain SSM4.2T belongs to the genus Flavobacterium and shares high-sequence similarity with F. limi KACC 18851T (98.1%), F. hydrophilum KACC 19591T (97.6%), F. defluvii KCTC 12612T (97.1%), F. cheongpyeongense KACC 19592T (97.0%) and F. fluviatile KCTC 52446T (96.9%). Strain SSM4.2T had 73.2–84.6% average nucleotide identity and 19.1–29.4% digital DNA-DNA hybridization values with its closest type strains. Based on its phenotypic, chemotaxonomic, phylogenetic and genomic features, strain SSM4.2T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium ajazii sp. nov. is proposed. The type strain is SSM4.2T (= KCTC 72807T = MCCC 1K04370T).

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Acknowledgements

This work was supported by the Natural Science Foundation of Zhejiang Province (LY18D060003). We would like to thank Dr. Xu Youping and Dr. Ge Zhiwei (Analysis Center of Agrobiology and Environmental Sciences, Faculty of Agriculture, Life and Environment Sciences, Zhejiang University) for fatty acid and respiratory quinones analysis, respectively

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  1. Ishrat Khan and Sanjit Chandra Debnath contributed equally to this work.

Authors and Affiliations

  1. Ocean College, Zhejiang University, Room 354, Marine Science Building, No1 Zheda Road, Dinghai District, Zhoushan, 316021, Zhejiang, China

    Ishrat Khan, Sanjit Chandra Debnath, Cen Yan, Can Chen, Yan Xu, Wen-Jie Wang, Yu-Chao Yu, Dao-Qiong Zheng, Jin-Zhong Xu & Pin-Mei Wang

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  1. Ishrat Khan
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Correspondence to Pin-Mei Wang.

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Khan, I., Debnath, S.C., Yan, C. et al. Flavobacterium ajazii sp. nov., Isolated from Seaweed of Gouqi Island, China. Curr Microbiol 77, 2925–2932 (2020). https://doi.org/10.1007/s00284-020-02112-1

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