Summary
Experiments were conducted to study the relationship between the production of interstrand crosslinks by 4,5′,8-trimethylpsoralen (psoralen) in simian virus 40 DNA and the ability of psoralen to inactivate the virus. Under conditions where only single viral particles enter a given host cell, approximately one crosslink was lethal to the virus and could not be repaired. In contrast, when multiple viral genomes infected a host cell, psoralen-induced crosslinks were repaired (multiplicity reactivation). A model is proposed for multiplicity reactivation which involves genetic recombination between damaged viral genomes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- DNA:
-
deoxyribonucleic acid
- SV40:
-
simian virus 40
- psoralen:
-
4,5′,8-trimethylpsoralen
- EDTA:
-
disodium ethylenediamine-tetraacetate
References
Ashwood-Smith MJ, Poulton GA, Barker M, Mildenberger M (1980) 5-methoxypsoralen, an ingredient in several suntan preparations, has lethal, mutagenic and clastogenic properties. Nature 285:407–409
Cohen LF, Kraemer KH, Waters HL, Kohn KW, Glaubiger DL (1981) DNA crosslinking and cell survival in human lymphoid cells treated with 8-methoxypsoralen and long wavelength ultraviolet radiation. Mutat Res 80:347–356
Cole RS (1971a) Psoralen monoadducts and interstrand cross-links in DNA. Biochim Biophys Acta 254:30–39
Cole RS (1971b) Inactivation of Escherichia coli, F′ episomes at transfer, and bacteriophage lambda by psoralen plus 360-nm light: Significance of deoxyribonucleic acid cross-links. J Bacteriol 107:846–852
Cole RS (1973) Repair of DNA containing interstrand crosslinks in Escherichia coli: Sequential excision and recombination. Proc Natl Acad Sci USA 70:1064–1068
Crawford LV, Cole CN, Smith AE, Paucha E, Tegtmeyer P, Rundell K, Berg P (1978) Organization and expression of early genes of simian virus 40. Proc Natl Acad Sci USA 75:117–121
Dasgupta UB, Summers WC (1980) Genetic recombination of herpes simplex virus, the role of the host cell and UV-irradiation of the virus. Mol Gen Genet 178:617–623
Day RS, Giuffrida AS, Dingman CW (1975) Repair by human cells of adenovirus-2 damaged by psoralen plus near ultraviolet light treatment. Mutat Res 33:311–320
Epstein JH, Farber EM (1979) Current status of oral PUVA therapy for psoriasis. J Am Acad Dermatol 1:106–117
Fujiwara Y, Tatsumi M, Sasaki MS (1977) Cross-link repair in human cells and its possible defect in Fanconi's anemia cells. J Mol Biol 113:635–649
Gilchrest BA (1979) Methoxsalen photochemotherapy for mycosis fungoides. Cancer Treat Rep 63:663–667
Hall JD, Tokuno S-i (1979) Enhanced transformation of xeroderma pigmentosum variant cells by ultraviolet light-irradiated simian virus 40. Cancer Res 39:4064–4068
Hall JD, Featherston JD, Almy RE (1980) Evidence for repair of ultraviolet light-damaged herpes virus in human fibroblasts by a recombination mechanism. Virology 105:490–500
Hall JD, Scherer K (1981) Repair of psoralen-treated DNA by genetic recombination in human cells infected with herpes simplex virus. Cancer Res 41:5033–5038
Hall JD, Almy RE, Scherer KL (1982) DNA repair in cultured human fibroblasts does not decline with donor age. Exp Cell Res 139:351–359
Huskey RJ (1969) Multiplicity reactivation as a test for recombination function. Science 164:319–320
Kaye J, Smith CA, Hanawalt PC (1980) DNA repair in human cells containing photoadducts of 8-methoxypsoralen or angelicin. Cancer Res 40:696–702
Khoury G, Lai C-J (1979) Preparation of simian virus 40 and its DNA. Methods Enzymol 58:404–412
Lai C-J, Nathans D (1974) Mapping the genes of simian virus 40. Cold Spring Harbor Symp Quant Biol 39:53–60
Lai C-J, Nathans D (1975) A map of temperature-sensitive mutants of simian virus 40. Virology 66:70–81
Maxam AM, Gilbert W (1980) Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol 65:499–560
Selsky CA, Henson P, Weichselbaum RR, Little JB (1979) Defective reactivation of ultraviolet light-irradiated herpes virus by a Bloom's syndrome fibroblast strain. Cancer Res 39:3392–3396
Vincent RA, Huang PC (1976) The proportion of cells labeled with tritiated thymidine as a function of population doubling level in cultures of fetal, adult, mutant and tumor origin. Exp Cell Res 102:31–42
Yamamoto H, Shimojo H (1971) Multiplicity reactivation of human adenovirus type 12 and simian virus 40 irradiated by ultraviolet light. Virology 45:529–531
Author information
Authors and Affiliations
Additional information
Communicated by G. O'Donovan
Rights and permissions
About this article
Cite this article
Hall, J.D. Repair of psoralen-induced crosslinks in cells multiply infected with SV40. Molec Gen Genet 188, 135–138 (1982). https://doi.org/10.1007/BF00333007
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00333007