Abstract
Meiosis is a specialized cell division in which two chromosome segregation phases follow a single DNA replication phase. The budding yeast Polo-like kinase Cdc5 was found to be instrumental in establishing the meiosis I chromosome segregation program. Cdc5 was required to phosphorylate and remove meiotic cohesin from chromosomes. Furthermore, in the absence of CDC5 kinetochores were bioriented during meiosis I, and Mam1, a protein essential for coorientation, failed to associate with kinetochores. Thus, sister-kinetochore coorientation and chromosome segregation during meiosis I are coupled through their dependence on CDC5.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, Non-P.H.S.
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Anaphase
-
Cdc20 Proteins
-
Cell Cycle Proteins / metabolism
-
Cell Nucleus / metabolism
-
Centromere / physiology
-
Chromatids / physiology
-
Chromosome Segregation*
-
Chromosomes, Fungal / physiology*
-
Kinetochores / physiology
-
Meiosis*
-
Metaphase
-
Nuclear Proteins / metabolism
-
Phosphorylation
-
Protein Kinases / genetics
-
Protein Kinases / metabolism*
-
Protein Serine-Threonine Kinases
-
Saccharomyces cerevisiae / enzymology
-
Saccharomyces cerevisiae / genetics
-
Saccharomyces cerevisiae / physiology*
-
Saccharomyces cerevisiae Proteins / genetics
-
Saccharomyces cerevisiae Proteins / metabolism
-
Securin
Substances
-
CDC20 protein, S cerevisiae
-
Cdc20 Proteins
-
Cell Cycle Proteins
-
Nuclear Proteins
-
PDS1 protein, S cerevisiae
-
Saccharomyces cerevisiae Proteins
-
Securin
-
Protein Kinases
-
Protein Serine-Threonine Kinases
-
CDC5 protein, S cerevisiae