The present paper deals with an attempt to find the satisfactory method to permit rapid growth of Artemia and to maintain their survival at a high ratio.
Dried eggs of Artemia obtained commercially were used for the material. Dried eggs were allowed to hatch in the artificial sea water (Table 1). The culture was started by inoculating 300 or so newborn larvae into 500ml of the culture medium. During the culture, the medium was incubated at 28°C and aerated 20ml per min., and fine ground food was added 100mg per 100 individuals every day.
The results obtained are summarized as follows:
1) The larvae hatched from dried eggs under aerated condition showed more successful results than those nnder stationary condition.
2) Artemia were allowed to grow rapidly to adult and multiply enormously in the culture nedium added NaCl and chelated metals to artificial sea water (Table 2).
3) The most suitable specific gravity of the medium was restricted to the range of about 1.04 (Fig. 1).
4) The addition of chelated metals caused raising the survival ratio, accelerating the speed of growth and increasing the egg production (Fig. 2).
5) The beneficial actions of chelated metals were mainly due to chelated iron and manganese (Fig. 3).
6) Baker's yeast was a good food, while “WAKAMOTO” composed of yeast and vitamin B complex materials in addition was the better food than the former (Fig. 4).
7) The acetone-butanol fermentation waste was a very good food from the point of view of practical utility (Fig. 5).
8) The newborn larvae grew to adults (at 50-65% survivals) and began to copulate after 6-7 days. The copulated females began to spawn after following 3-4 days. The number of eggs produced by a female was 100 on an average. The hatching out of the fresh eggs took place within several hours in the culture medium (Table 3).