書誌事項
- タイトル別名
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- BASIC CHARACTERISTICS OF A NOVEL DIAGNOSTIC METHOD (QuantiFERON TB-2G) OF LATENT TUBERCULOSIS INFECTION WITH A USE OF MYCOBACTERIUM TUBERCULOSIS-SPECIFIC ANTIGENS, ESAT-6 AND CFP-10
- ケッカクキン コウゲン ESAT 6 オヨビ CFP 10 オ モチイタ ケッカク カンセン シンダンホウ QuantiFERON TB 2G ノ キソテキ ケントウ
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抄録
[Purposes] To determine the optimum cut-off level of a newly developed method for diagnosing tuberculosis infection based on whole-blood interferon-gamma measurement, and to study the basic characteristics of the method.<BR>[Study Subjects] 1) A total of 220 young, healthy individuals having no apparent exposure to tuberculosis infection, most of whom have had a vaccination with BCG vaccine. 2) One hundred eighteen tuberculosis patients who were diagnosed by positive Mycobacterium tuberculosis on culture. 3) A group of 75 youngsters exposed to an infectious tuberculosis patient and who showed a strong tuberculin reaction (with erythema diameter of 30 mm or more).<BR>[Method] Whole-blood specimens of donors were stimulated with antigens, i.e., ESAT-6 and CFP-10, and then cultured. Plasma concentrations of interferon-gamma discharged were then determined with QuantiFERON ®-CMI. Correlation between interferon-gamma concentrations in response to ESAT-6 and CFP-10, and their correlation with Mantoux test results were analyzed for various categories of donors. The Receiver Operating Characteristics analysis was performed considering the loss due to misclassification.<BR>[Results and Discussion] The optimum cut-off level was determined as 0.35 IU/ml for both ESAT-6 and CFP-10. This gave the test a sensitivity of 89.0% and specificity of 98.1% in detecting tuberculosis infection. The correlation of interferongamma response with tuberculin tests among BCG-vaccinated individuals was low, which suggested that the test was not influenced by previous BCG vaccination. The low correlation between ESAT-6 and CFP-10 tests suggested that the simultaneous use of the two tests was beneficial. As in the case of clinical tests in general, the cut-off should be set at a lower level when the test is applied to high prevalence situation and vice versa.
収録刊行物
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- 結核
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結核 79 (12), 725-735, 2004
一般社団法人 日本結核病学会
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詳細情報 詳細情報について
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- CRID
- 1390282679978129280
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- NII論文ID
- 10014466582
- 10019732775
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- NII書誌ID
- AN00073442
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- COI
- 1:CAS:528:DC%2BD2MXjtVGitLw%3D
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- ISSN
- 18842410
- 00229776
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- NDL書誌ID
- 7268962
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- PubMed
- 15782618
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可