Abstract
The recent explosion in genomic sequencing has made available a wealth of data that can now be analyzed to identify protein antigens, potential targets for vaccine development. Here we present, in the context of Plasmodium falciparum, a strategy that rapidly identifies target antigens from large and complex genomes. Sixteen antigenic proteins recognized by volunteers immunized with radiation-attenuated P. falciparum sporozoites, but not by mock immunized controls, were identified. Several of these were more antigenic than previously identified and well characterized P. falciparum-derived protein antigens. The data suggest that immune responses to Plasmodium are dispersed on a relatively large number of parasite antigens. These studies have implications for our understanding of immunodominance and breadth of responses to complex pathogens.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Adult
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Algorithms
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Alleles
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Animals
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Antigens, Protozoan / genetics*
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Antigens, Protozoan / isolation & purification
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Genes, Protozoan
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Genome, Protozoan
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Genomics
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Humans
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Immunization
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In Vitro Techniques
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Interferon-gamma / biosynthesis
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Malaria Vaccines / genetics
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Malaria Vaccines / immunology
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Malaria Vaccines / isolation & purification
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Male
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Middle Aged
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Molecular Sequence Data
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Plasmodium falciparum / genetics*
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Plasmodium falciparum / immunology*
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Proteomics
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Protozoan Proteins / genetics
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Protozoan Proteins / immunology
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Protozoan Proteins / isolation & purification
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T-Lymphocytes / immunology
Substances
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Antigens, Protozoan
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Malaria Vaccines
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Protozoan Proteins
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Interferon-gamma
Associated data
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GENBANK/PF100179
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GENBANK/PF110226
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GENBANK/PF110341
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GENBANK/PF110435
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GENBANK/PF110456
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GENBANK/PF110479
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GENBANK/PF130278
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GENBANK/PF130320
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GENBANK/PF140074
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GENBANK/PF140236
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GENBANK/PF140291
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GENBANK/PF140372
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GENBANK/PF140648
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GENBANK/PF140751