Studies have shown significant coronal dye and bacterial leakage following exposure of sealed root canals to artificial and natural saliva. The purpose of this study was to determine the time needed for bacteria in natural saliva to contaminate the entire length of root canals obturated by lateral and vertical condensation techniques. Forty root canals were cleaned and shaped using a step-back technique. Thirty root canals were obturated with gutta-percha and root canal sealer using either lateral or vertical condensation techniques. Forty root canals were obturated without a root canal sealer and served as positive controls. After obturation, the coronal 3 mm of five root canals were sealed with sticky wax and served as negative controls. The coronal portions of the filling materials were placed in contact with human saliva and the number of days required for bacteria in saliva to penetrate the entire root canals were determined. No bacterial leakage occurred in the negative control group. Complete bacterial leakage occurred within 2 days in the positive control group. All root canals were recontaminated in less than 30 days. No statistical significant difference was found between the two methods of obturation.