Abstract
Pre-B lymphocytes, and hybridomas derived from them, synthesize immunoglobulin heavy (IgH) chain in the absence of light (L) chain. In the Abelson virus transformed line 18-81, which is representative of the pre-B cell stage, we observed that at least some of the H-chains are bound to a protein other than L-chain. Here we show that the protein (which we term immunoglobulin heavy-chain binding protein, BiP) binds non-covalently to free IgH, but not to IgH associated with IgL.
MeSH terms
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Animals
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B-Lymphocytes / metabolism
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Carrier Proteins / isolation & purification
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Carrier Proteins / metabolism*
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Endoplasmic Reticulum Chaperone BiP
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Heat-Shock Proteins*
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Immunoglobulin Heavy Chains / metabolism*
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Immunoglobulin Light Chains / metabolism
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Mice
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Molecular Chaperones*
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Molecular Weight
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Myeloma Proteins / analysis
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Peptide Fragments / analysis
Substances
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Carrier Proteins
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Endoplasmic Reticulum Chaperone BiP
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Heat-Shock Proteins
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Immunoglobulin Heavy Chains
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Immunoglobulin Light Chains
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Molecular Chaperones
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Myeloma Proteins
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Peptide Fragments