Abstract
C57BL/6 mice transgenic for a mu heavy chain gene, the VDJ region of which came from the BALB/c hybridoma 17.2.25, expressed high levels of antibody carrying determinants specific for the transgene (idiotypes). The individual antibodies made by hybridomas from transgenic mice, however, were generally encoded by endogenous genes; in most cases the transgene was present but not expressed. The endogenous, idiotype-positive antibodies had heavy chains that were notable for the high frequencies of JH4 (as in the transgene) and VH segments from the VH81X family (unrelated to the transgene). The expression of endogenous genes mimicking the idiotype of the transgene suggests that a rearranged gene introduced into the germ line can activate powerful cellular regulatory influences.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
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Retracted Publication
MeSH terms
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Animals
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Antibodies, Monoclonal / immunology
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DNA / genetics
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Female
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Gene Expression Regulation*
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Hybridomas / immunology
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Immunoglobulin Heavy Chains / biosynthesis
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Immunoglobulin Heavy Chains / genetics*
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Immunoglobulin Idiotypes / genetics
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Immunoglobulin mu-Chains / biosynthesis
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Immunoglobulin mu-Chains / genetics*
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Male
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Mice
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Mice, Inbred BALB C / genetics*
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Mice, Inbred BALB C / immunology
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Mice, Inbred C57BL / genetics*
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Mice, Inbred C57BL / immunology
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Microinjections
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Molecular Sequence Data
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / genetics
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Transformation, Genetic
Substances
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Antibodies, Monoclonal
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Immunoglobulin Heavy Chains
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Immunoglobulin Idiotypes
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Immunoglobulin mu-Chains
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Recombinant Proteins
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DNA
Associated data
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GENBANK/M13328
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GENBANK/M13329
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GENBANK/M13330
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GENBANK/M13331
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GENBANK/M13332
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GENBANK/M13333
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GENBANK/M13334