Objective: To develop an HPLC method to determine Puerarin, Daidzin and Daidzein in Pueraria lobata.
Method: The separation was performed in a SYMMETRY C18 column with a mobile phase of methanol-1% HAC solution (gradient elution), the The detection wavelength being 250 nm.
Result: The average recovery was respectively 101.7%, 100.7% and 101.7% (n = 3), RSD 0.43%, 0.82% and 1.50% (n = 3) for Puerarin, Daidzin and Daidzein.
Conclusion: This method is suitable for the determination of Puerarin, Daidzin and Daidzein in Pueraria lobata and its preparation.