To establish a way to produce nematode- resistant Japanese black pine (Pinus thunbergii Parl.) without the artificial inoculation of pine wood nematodes (Bursaphelenchus xylophilus Nickle), ortets that were healthy after an inoculation test were subjected to rooted cutting. The average percentage of rooted cuttings was 25.6%. When the temperature of the rooting media was raised using an electrically heated bed, the percentage of rooted cuttings was improved significantly, from 35.5% in the control group to 54.7% on average. Annual cuttings were taken from the same ortets, which ranged in age from 3 to 7 years, to study the relationship between ortet age and percentage of rooted cuttings. The average percentage of rooted cuttings remained between 25.6 and 47.9%, irrespective of ortet age. This may have resulted from reinvigora- tion caused by cutting and pruning, which prevented an aging effect. Since the percentages of rooted cuttings from each individual were significantly related between years, the difficulty in obtaining rooted cuttings was determined genetically. Clonal plantlets produced by cutting from the ortets that were healthy after the inoculation test were inoculated artificially. On average, 90.3% survived inoculation, but only 51.6% were healthy. This paper examines the usefulness of this cutting propagation technique for producing nematode-resistant Japanese black pine plantlets.