Abstract
Members of the casein kinase 1 family of serine/threonine kinases are highly conserved from yeast to mammals and seem to play an important role in vesicular trafficking, DNA repair, cell cycle progression and cytokinesis. We here report that in interphase cells of various mammalian species casein kinase 1 delta (CK1delta) specifically interacts with the trans Golgi network and cytoplasmic, granular particles that associate with microtubules. Furthermore, at mitosis CK1delta is recruited to the spindle apparatus and the centrosomes in cells, which have been exposed to DNA-damaging agents like etoposide or gammairradiation. In addition, determination of the affinity of CK1delta to different tubulin isoforms in immunoprecipitation-Western analysis revealed a dramatically enhanced complex formation between CK1delta and tubulins from mitotic extracts after introducing DNA damage. The high affinity of CK1delta to the spindle apparatus in DNA-damaged cells and its ability to phosphorylate several microtubule-associated proteins points to a regulatory role of CK1delta at mitosis.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
3T3 Cells
-
Animals
-
Antineoplastic Agents / pharmacology
-
Antineoplastic Agents, Phytogenic / pharmacology
-
Casein Kinases
-
Cell Cycle / drug effects
-
Cell Cycle / radiation effects
-
Cell Line
-
Cells, Cultured
-
Clathrin / metabolism
-
Cytoplasm / metabolism
-
Cytoskeleton / metabolism
-
DNA Damage / drug effects
-
DNA Damage / radiation effects
-
Etoposide / pharmacology
-
Gamma Rays
-
Glutathione Transferase / metabolism
-
Golgi Apparatus / metabolism*
-
Mice
-
Mice, Inbred BALB C
-
Microtubule Proteins*
-
Microtubule-Associated Proteins / metabolism
-
Microtubules / metabolism*
-
Mitosis / drug effects
-
Mitosis / radiation effects
-
Nocodazole / pharmacology
-
Nucleic Acid Synthesis Inhibitors / pharmacology
-
Phosphoproteins / metabolism
-
Protein Isoforms / metabolism
-
Protein Kinases / metabolism*
-
Spindle Apparatus / drug effects
-
Spindle Apparatus / metabolism*
-
Spindle Apparatus / radiation effects
-
Stathmin
-
Tubulin / chemistry
-
Tubulin / metabolism
-
Vinblastine / pharmacology
-
tau Proteins / metabolism
Substances
-
Antineoplastic Agents
-
Antineoplastic Agents, Phytogenic
-
Clathrin
-
Microtubule Proteins
-
Microtubule-Associated Proteins
-
Nucleic Acid Synthesis Inhibitors
-
Phosphoproteins
-
Protein Isoforms
-
Stathmin
-
Stmn1 protein, mouse
-
Tubulin
-
tau Proteins
-
Vinblastine
-
Etoposide
-
Glutathione Transferase
-
Protein Kinases
-
Casein Kinases
-
Nocodazole