Abstract
HYBRIDS between two distinct differentiated cell types usually do not express the differentiated characteristics of either parental cell. The synthesis of enzymes necessary for the metabolism of the cell continues, while the synthesis of specialized products of differentiation often is suppressed1. Mouse myeloma cells, after fusion with mouse fibroblasts, follow this pattern ; that is, the hybrid cells cease synthesis of immunoglobulin2,3. A myeloma × lymphoma hybrid, however, continues secretion of immunoglobulin4,5. We report here the isolation of a hybrid clone, resulting from fusion of mouse myeloma cells secreting immunoglobulin of known specificity and human peripheral blood lymphocytes not secreting detectable immunoglobulin. The hybrid cells continue secretion of mouse immunoglobulin and initiate synthesis and secretion of human immunoglobulin.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Ephrussi, B., in Hybridization of Somatic Cells, 53 (Princeton University Press, Princeton, 1972).
Coffino, P., Knowles, B., Nathenson, S. G., and Scharff, M. D., Nature new Biol., 231, 84 (1971).
Periman, P., Nature, 228, 1087 (1970).
Mohit, B., and Fan, K., Science, N.Y., 171, 75 (1971).
Mohit, B., Proc. natn. Acad. Sci. U.S.A. 68, 3045 (1971).
Potter, M., and Lieberman, R., J. exp. Med., 132, 737 (1970).
Klebe, R. J., Chen, T., and Ruddle, F., J. Cell Biol., 45, 74 (1970).
Moorhead, P. S., Nowell, P. C., Mellman, W., Battips, D., and Hungerford, D., Expl. Cell Res., 20, 613 (1960).
Seabright, M., Lancet, ii, 971 (1971).
Maizel, J., in Fundamental Techniques in Virology (edit. by Habel, K., and Salzman, N.) (Academic Press, New York, 1969).
Von Furth, R., Schuit, H., and Higmans, W., Immunology, 11, 1 (1966).
Ephrussi, B., Hybridization of Somatic Cells, 28 (Princeton University Press, 1972).
Migeon, B. R., and Miller, C. S., Science, N.Y., 162, 1005 (1968).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
SCHWABER, J., COHEN, E. Human × Mouse Somatic Cell Hybrid Clone secreting Immunoglobulins of both Parental Types. Nature 244, 444–447 (1973). https://doi.org/10.1038/244444a0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/244444a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
