Use of synthetic oligonucleotides as hybridization probes: isolation of cloned cDNA sequences for human beta 2-microglobulin

Proc Natl Acad Sci U S A. 1981 Nov;78(11):6613-7. doi: 10.1073/pnas.78.11.6613.

Abstract

We have synthesized two sets of 15-base-long oligodeoxyribonucleotides corresponding to all possible coding sequences for a small portion of human beta 2-microglobulin. Labeled oligonucleotides were used as hybridization probes to screen bacterial clones containing cDNA sequences primed with oligo(dT) and inserted into the plasmid vector pBR322. One beta 2-microglobulin cDNA clone was detected in the 535 bacterial plasmid clones that were screened. The clone has been characterized by blotting and nucleotide sequence analysis. The cloned beta 2-microglobulin sequence contains 217 base pairs of the 3' untranslated region of the mRNA and 328 base pairs (97%) of the coding region.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Beta-Globulins / genetics*
  • Cloning, Molecular*
  • DNA, Recombinant / isolation & purification*
  • Escherichia coli / genetics
  • Humans
  • Nucleic Acid Hybridization
  • Oligodeoxyribonucleotides / chemical synthesis*
  • Oligonucleotides / chemical synthesis*
  • Plasmids
  • beta 2-Microglobulin / genetics*

Substances

  • Beta-Globulins
  • DNA, Recombinant
  • Oligodeoxyribonucleotides
  • Oligonucleotides
  • beta 2-Microglobulin

Associated data

  • GENBANK/J00105