Abstract
The Eph-related receptor tyrosine kinase gene, Cek-8, is expressed in mesenchyme at the tip of chick limb buds, with high levels of transcripts posteriorly and apically but fading out anteriorly. Expression of Cek-8 in distal mesenchyme is regulated by apical ridge- and FGF-polarising signals and retinoic acid, and is uniform across the anteroposterior axis in talpid3 mutants. These data indicate that Cek-8 expression responds to regulatory signals during limb patterning and suggest that this receptor tyrosine kinase may have a role in coordinating responses to signals in the progress zone of early buds. Later on in limb development, Cek-8 expression is associated with cell condensations that form tendons and their attachments to cartilage rudiments and then in developing feather buds.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bone Morphogenetic Proteins
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Cell Communication
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Chick Embryo
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Feathers / embryology
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Fibroblast Growth Factor 2 / pharmacology
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Gene Expression Regulation, Developmental / drug effects
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Gene Expression Regulation, Developmental / physiology*
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Growth Substances / pharmacology
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Limb Buds / cytology
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Limb Buds / growth & development
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Limb Buds / physiology*
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Mesoderm / chemistry
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Mice
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Mice, Inbred C57BL
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Microspheres
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Mutation
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Neuropeptides / genetics*
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Proteins / pharmacology
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RNA, Messenger / analysis
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Receptor Protein-Tyrosine Kinases / genetics*
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Receptor, EphA4
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Tendons / chemistry
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Tendons / embryology
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Tretinoin / pharmacology
Substances
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Bone Morphogenetic Proteins
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Growth Substances
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Neuropeptides
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Proteins
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RNA, Messenger
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Fibroblast Growth Factor 2
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Tretinoin
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Receptor Protein-Tyrosine Kinases
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Receptor, EphA4