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Paenibacillus polymyxa Rhizobacteria and Their Synthesized Exoglycans in Interaction with Wheat Roots: Colonization and Root Hair Deformation

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Abstract

We examined the ability of several Paenibacillus polymyxa strains to colonize wheat roots and the ability of P. polymyxa exoglycans to induce root hair deformation. For the first time, exopolysaccharides isolated from P. polymyxa were found to produce, with different intensities, various morphological changes in the root hairs of wheat seedlings, which are some of the earliest responses of plants to bacteria in the surrounding milieu. P. polymyxa 1465, giving the highest exopolysaccharide yield and the highest viscosity of aqueous exopolysaccharide solutions, was best able to colonize wheat seedling roots, and its exopolysaccharide proved to be the best in producing root hair deformation. It is suggested that P. polymyxa exoglycans have an active role in the establishment of plant–microbe associations.

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References

  1. Alami Y, Achouak W, Marol C et al (2000) Rhizosphere soil aggregation and plant growth promotion of sunflowers by an exopolysaccharide-producing Rhizobium sp. strain isolated from sunflower roots. Appl Environ Microbiol 66:3393–3398

    Article  CAS  PubMed  Google Scholar 

  2. Amellal N, Burtin G, Bartoli F et al (1998) Colonization of wheat roots by an exopolysaccharide-producing Pantoea agglomerans strain and its effect on rhizosphere soil aggregation. Appl Environ Microbiol 64:3740–3747

    CAS  PubMed  Google Scholar 

  3. Baldani VLD, Baldani JI, Döbereiner J (1983) Effects of Azospirillum inoculation on root infection and nitrogen incorporation in wheat. Can J Microbiol 29:924–929

    Article  Google Scholar 

  4. Bezzate S, Aymerich S, Chambert R et al (2000) Disruption of the Paenibacillus polymyxa levansucrase gene impairs its ability to aggregate soil in the wheat rhizosphere. Environ Microbiol 2:333–342

    Article  CAS  PubMed  Google Scholar 

  5. Bianciotto V, Andreotti S, Balestrini R et al (2001) Mucoid mutants of the biocontrol strain Pseudomonas fluorescens CHA0 show increased ability in biofilm formation on mycorrhizal and nonmycorrhizal carrot roots. Mol Plant Microbe Interact 14:255–260

    Article  CAS  PubMed  Google Scholar 

  6. Boyko AS, Konnova SA, Fedonenko YuP et al (2011) Structural and functional peculiarities of the lipopolysaccharide of Azospirillum brasilense SR55 isolated from the roots of Triticum durum. Microbiol Res 166:585–593

    Article  CAS  PubMed  Google Scholar 

  7. Chenu C (1995) Extracellular polysaccharides: an interface between microorganisms and soil constituents. In: Huang PM, Berthelin J, Bollag JM et al (eds) Environmental impact of soil component interactions. CRC Lewis Publishers, Boca Raton, pp 217–233

    Google Scholar 

  8. Dische Z (1962) Color reactions of hexuronic acids. Methods Carbohydr Chem 1:497–501

    CAS  Google Scholar 

  9. Dubois M, Gilles KA, Hamilton JK et al (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356

    Article  CAS  Google Scholar 

  10. Fåhraeus G (1957) The infection of clover root hairs by nodule bacteria studied by simple glass slide technique. J Gen Microbiol 16:374–381

    PubMed  Google Scholar 

  11. Fedonenko YuP, Yegorenkova IV, Konnova SA et al (2001) Involvement of the lipopolysaccharides of Azospirilla in the interaction with wheat seedling roots. Microbiology (Moscow, Russ Fed) 70:329–334

    Google Scholar 

  12. Fujishige NA, Kapadia NN, De Hoff PL et al (2006) Investigations of Rhizobium biofilm formation. FEMS Microbiol Ecol 56:195–206

    Article  CAS  PubMed  Google Scholar 

  13. Haggag WM (2007) Colonization of exopolysaccharide-producing Paenibacillus polymyxa on peanut roots for enhancing resistance against crown rot disease. Afr J Biotechnol 6:1568–1577

    CAS  Google Scholar 

  14. Haggag WM (2010) The role of biofilm exopolysaccharides on biocontrol of plant disease. In: Elnashar M (ed) Biopolymers, vol. 14. pp 271–284

  15. Halverson LJ, Stacey G (1986) Signal exchange in plant–microbe interactions. Microbiol Rev 50:193–225

    CAS  PubMed  Google Scholar 

  16. Jain DK, Patriquin DG (1984) Root hair deformation, bacterial attachment, and plant growth in wheat-Azospirillum associations. Appl Environ Microbiol 48:1208–1213

    CAS  PubMed  Google Scholar 

  17. Kaci Y, Heyraud A, Barakat M et al (2005) Isolation and identification of an EPS-producing Rhizobium strain from arid soil (Algeria): characterization of its EPS and the effect of inoculation on wheat rhizosphere soil structure. Res Microbiol 156:522–531

    Article  CAS  PubMed  Google Scholar 

  18. Karpunina LV (2002) Rol’ agglyutiniruyushchikh belkov azotfiksiruyushchikh batsill i rizobii v zhiznedeyatel’nosti bakterii pri vzaimodeistvii s rasteniyami (Role of agglutinative proteins of nitrogen-fixing bacilli and rhizobia in the vital functions of the bacteria during their interaction with plants). Dr. Sci. (Biol.) Dissertation, Saratov (in Russian)

  19. Konnova SA, Skvortsov IM, Makarov OE et al (1995) Polysaccharide complexes secreted by Azospirillum brasilense and their possible role in the interaction of bacteria with wheat roots. Mikrobiologiya 64:762–768 (in Russian)

    CAS  Google Scholar 

  20. Matthysse AG, Marry M, Krall L et al (2005) The effect of cellulose overproduction on binding and biofilm formation on roots by Agrobacterium tumefaciens. Mol Plant Microbe Interact 18:1002–1010

    Article  CAS  PubMed  Google Scholar 

  21. Michiels KW, Croes CL, Vanderleyden J (1991) Two different modes of attachment of Azospirillum brasilense Sp7 to wheat roots. J Gen Microbiol 137:2241–2246

    CAS  Google Scholar 

  22. Morris CE, Monier J-M (2003) The ecological significance of biofilm formation by plant-associated bacteria. Annu Rev Phytopathol 41:429–453

    Article  CAS  PubMed  Google Scholar 

  23. Petersen DJ, Srinivasan M, Chanway CP (1996) Bacillus polymyxa stimulates increased Rhizobium etli populations and nodulation when co-resident in the rhizosphere of Phaseolus vulgaris. FEMS Microbiol Lett 142:271–276

    Article  CAS  PubMed  Google Scholar 

  24. Santaella C, Schue M, Berge O et al (2008) The exopolysaccharide of Rhizobium sp. YAS34 is not necessary for biofilm formation on Arabidopsis thaliana and Brassica napus roots but contributes to root colonization. Environ Microbiol 10:2150–2163

    Article  CAS  PubMed  Google Scholar 

  25. Scopes RK (1982) Protein purification: principles and practice. Springer, New York

    Google Scholar 

  26. Slonecker JH (1972) Gas–liquid chromatography of alditol acetates. Methods Carbohydr Chem 6:20–24

    Google Scholar 

  27. Tien TM, Gaskins MN, Hubbell DH (1979) Plant growth substances produced by Azospirillum brasilense and their effect on the growth of pearl millet (Pennisetum americanum L.). Appl Environ Microbiol 37:1016–1024

    CAS  PubMed  Google Scholar 

  28. Timmusk S, Grantcharova N, Wagner EGH (2005) Paenibacillus polymyxa invades plant roots and forms biofilms. Appl Environ Microbiol 71:7292–7300

    Article  CAS  PubMed  Google Scholar 

  29. Yegorenkova IV, Konnova SA, Sachuk VN et al (2001) Azospirillum brasilense colonisation of wheat roots and the role of lectin–carbohydrate interactions in bacterial adsorption and root-hair deformation. Plant Soil 231:275–282

    Article  CAS  Google Scholar 

  30. Yegorenkova IV, Tregubova KV, Matora LYu et al (2010) Use of ELISA with antiexopolysaccharide antibodies to evaluate wheat-root colonization by the rhizobacterium Paenibacillus polymyxa. Curr Microbiol 61:376–380

    Article  CAS  PubMed  Google Scholar 

  31. Yegorenkova IV, Tregubova KV, Matora LYu (2011) Biofilm formation by Paenibacillus polymyxa strains differing in the production and rheological properties of their exopolysaccharides. Curr Microbiol 62:1554–1559

    Article  CAS  PubMed  Google Scholar 

  32. York GM, González JE, Walker GC (1996) Exopolysaccharides and their role in nodule invasion. In: Stacey G, Mullin B, Gresshoff PM (eds) Biology of molecular plant–microbe interactions. International Society for Molecular Plant–Microbe Interactions, St. Paul, Minn., USA, pp 325–330

  33. Zamudio M, Bastarrachea F (1994) Adhesiveness and root hair deformation capacity of Azospirillum strains for wheat seedlings. Soil Biol Biochem 26:791–797

    Article  Google Scholar 

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Acknowledgments

Our thanks go to Mr. Dmitry N. Tychinin (this institute) for translating the original manuscript into English.

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Authors and Affiliations

  1. Laboratory of Biochemistry, Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences (IBPPM RAS), 13 Prospekt Entuziastov, Saratov, 410049, Russian Federation

    Irina V. Yegorenkova, Kristina V. Tregubova & Vladimir V. Ignatov

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  1. Irina V. Yegorenkova
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  2. Kristina V. Tregubova
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  3. Vladimir V. Ignatov
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Correspondence to Irina V. Yegorenkova.

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Yegorenkova, I.V., Tregubova, K.V. & Ignatov, V.V. Paenibacillus polymyxa Rhizobacteria and Their Synthesized Exoglycans in Interaction with Wheat Roots: Colonization and Root Hair Deformation. Curr Microbiol 66, 481–486 (2013). https://doi.org/10.1007/s00284-012-0297-y

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