Design of a Novel Globular Protein Fold with Atomic-Level Accuracy
Abstract
A major challenge of computational protein design is the creation of novel proteins with arbitrarily chosen three-dimensional structures. Here, we used a general computational strategy that iterates between sequence design and structure prediction to design a 93-residue α/β protein called Top7 with a novel sequence and topology. Top7 was found experimentally to be folded and extremely stable, and the x-ray crystal structure of Top7 is similar (root mean square deviation equals 1.2 angstroms) to the design model. The ability to design a new protein fold makes possible the exploration of the large regions of the protein universe not yet observed in nature.
- Publication:
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Science
- Pub Date:
- November 2003
- DOI:
- 10.1126/science.1089427
- Bibcode:
- 2003Sci...302.1364K
- Keywords:
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- BIOCHEM