RNA was synthesized with isolated rat liver nuclei. Fingerprint analysis of
in vitro synthesized 5S RNA, which was slightly larger than mature 5S RNA on gel, showed that it contained all the sequence of mature 5S RNA and an additional sequence of about 8 nucleotides at its 3'-terminal site. The same molecule was also demonstrated
in vivo by a pulse-chase experiment, suggesting that 5S RNA is synthesized
in vivo as the larger precursor such as demonstrated in the
in vitro system.
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